The use of weak acid for differentiation allows excess stain to be removed from tissues, but will not remove stain from the acidfast organisms. Used in the demonstration of acidfast bacteria belonging to the genus mycobacterium, which include the causative agent for tuberculosis. Acid fast stain kinyoun cold method principle, procedure and result interpretation used for mycobacterium and parasites such as cryptosporidium. It is a variation of a method developed by robert koch in 1882. Murray pr, baron e, pfaller m, tenover f, yolken, eds. Joseph james joe kinyoun was born november 25, 1860 in east bend, north carolina, the oldest of five children born to elizabeth ann conrad and john hendricks kinyoun. We regularly produce chemical solutions to specifications designed by government and regulatory bodies, commercial and trade associations, and the specific needs of individual users and businesses. His family settled in post oak, missouri in 1866 after his house burned down during the civil war.
The lipoid capsule of the acidfast organism takes up carbolfuchsin and resists decolorization with a dilute acid rinse. Our product line consists of chemical solutions prepared to exact quality standards and certified for use in laboratories and production processes. Phenol is employed to render the cell walls of bacteria permeable to the fuchsin stain. Kinyoun carbol fuchsin download pdf learn how to make kinyoun carbol fuchsin and use it to stain acidfast organisms. Kinyoun, is a procedure used to stain acidfast species of the bacterial genera mycobacterium and nocardia and the apicomplexan genus cryptosporidium. Carbolfuchsin solution kinyoun, for staining acidfast. Kinyoun carbolfuschsin has a greater concentration of phenol and basic fuchsin and does not require heating in order to stain properly. If the pdf does not display below, you may also download it. At the age of 16, he studied medicine with his father, john hendricks kinyoun, who was a general practitioner. This file is licensed under the creative commons attributionshare alike 4. Kinyoun staining method uses carbolfuchsin as a primary stain, followed by decolorization with an acidalcohol solution and methylene blue as a counterstain. Tincion simple y tincion diferencial free download pdf ebook. Laura marcela gomez narvaez ivonne yessenia henao betancourth laura vanesa henao escobar resumen introduccion. Suggested by haawk for a 3rd party monetize your music today.
612 923 921 969 574 146 1228 1197 748 1332 414 735 86 649 568 838 598 1124 1079 261 873 204 197 1237 335 425 637 140 490 1375 58 301 311 408 661 1105 45 271 716 128 955